Western blot cost isn’t as fun to think about as your next research project, but it is a consideration in your lab budget. Considering that most research dollars are limited it is important to spend those precious resources wisely, and to avoid costly mistakes when conducting your experiments. In this article we will explore some common issues that can impact your western blot cost and solutions for avoiding these errors.

Western blotting is a complex technique which requires precise execution of multiple steps to generate accurate quantitative data. This includes proper gel separation of protein samples and selecting a membrane that will work well with your antibody, as well as optimal loading of high and low molecular weight proteins and transfer of the blot to nitrocellulose or PVDF. In addition, the sensitivity of chemiluminescent detection must be optimized and any non-specific signal should be minimized by using the best blocking reagents and proper incubation times.

Western Blot Cost Breakdown: How Much Should You Expect to Pay

Lastly, researchers must also ensure that the antibodies they use are correctly validated for their tissues of interest. Many antibody datasheets do not provide complete sample preparation guidelines, tissue specific information for the antibody or adequate negative controls which can significantly impact your western blot results. Inaccurate lane to lane normalization by housekeeping proteins or total protein can also lead to inaccurate western blot results. However, these errors are easy to correct if you know what to look for.…